Wednesday, November 27, 2019

Profile of Ernst Stromer the Famous Paleontologist

Profile of Ernst Stromer the Famous Paleontologist Born into an aristocratic German family on 1870, Ernst Stromer von Reichenbach achieved fame shortly before World War I, when he participated in a fossil-hunting expedition to Egypt. His Famous Discovery In the course of a few weeks, from January to February of 1911, Stromer identified and unearthed a series of large bones buried deep in the Egyptian desert, which challenged his paleontological skills (as he wrote in his journal, I don’t know how to conserve such massive species.) After carting the bones back to Germany, he stunned the world by announcing the discovery of a new genus of    sauropod, Aegyptosaurus, and two huge theropods, Carcharodontosaurus and the bigger than a T Rex,  Spinosaurus. Unfortunately, subsequent world events were not kind to Ernst Stromer. All of his hard-won fossils were destroyed during a raid by the Royal Air Force on Munich in 1944, during World War II, and two of his three sons died while serving in the German army. There is a bit of a happy ending, though: his third son, presumed dead, had actually been held a prisoner in the Soviet Union, and he was repatriated to Germany in 1950, two years before his father’s death. Stromer died in 1952.

Saturday, November 23, 2019

Learn ESL Through Movement

Learn ESL Through Movement If youve tried, and struggled, to learn English as a second language (ESL) the usual ways, its time to try it Dr. James Ashers way- through movement. With a student seated on each side of him, Asher demonstrates his technique by asking them to do what he does. Thats all. They dont repeat what he says, they just do what he does. Stand,  he says, and he stands. They stand. Walk, Asher says, and he walks. They walk. Turn. Sit. Point. Within minutes, he gives commands as complicated as, Walk to the chair and point at the table, and his students can do it by themselves. Heres the clincher. In his DVD, he demonstrates in Arabic, a language nobody in the room knows. In study after study, Asher has found that students of all ages can learn a new language quickly and stress-free in just 10-20 hours of silence. Students simply listen to a direction in the new language and do what the instructor does. Asher says, After understanding a huge chunk of the target language with TPR, students spontaneously begin to speak. At this point, students reverse roles with the instructor and utter directions to move their classmates and the instructor. Voila. Asher is the originator of the Total Physical Response approach to learning any language. His book, Learning Another Language Through Actions, is in its sixth edition. In it, Asher describes how he discovered the power of learning languages through physical movement, and the lengths to which he went to prove the technique through scientific experimentation involving the differences between the right and left brain. Ashers studies have proven that while the left brain puts up a fight against the memorization of new languages that occurs in so many classrooms, the right brain is completely open to responding to new commands, immediately. He is adamant about the need to comprehend a new language silently, by simply responding to it, before attempting to speak it, much like a new child imitates his or her parents before beginning to make sounds. While the book is on the academic side, and a little dry, it includes Ashers fascinating research, a lengthy and comprehensive QA that covers questions from both teachers and students, a directory of TPR presenters around the world, comparisons to other techniques, and get this, 53 lesson plans. Thats right- 53! He walks you through how to teach TPR in 53 specific sessions. Can learning take place if the students remain in their seats? Yes. Sky Oaks Productions, publisher of Ashers work, sells wonderful full-color kits of different settings such as home, airport, hospital, supermarket, and playground. Think Colorforms. Remember the pliable plastic forms that stick on a board and easily peel off to move? Responding to imperatives with these kits has the same result as physically moving. Asher also shares samples of mail he has received from people around the world. One of his letters is from Jim Baird, who writes that his classroom has wall-to-wall white boards on which he has created communities and complete countries. Baird writes: Students are required to drive, walk (with their fingers), fly, hop, run, etc. between buildings or cities, pick up things or people and deliver them to other places. They can fly into an airport and rent a car and drive it to another city where they can catch a flight or a boat, all kinds of possibilities. Sure is fun! Asher is generous with the materials and information he provides on his Sky Oaks Productions website, known as TPR World. He is clearly passionate about his work, and its easy to see why.

Thursday, November 21, 2019

The Success Story of Toyota Comapny in Qatar Essay

The Success Story of Toyota Comapny in Qatar - Essay Example Current paper focuses on the performance of Toyota in Qatar. Toyota is one of the major competitors in the global automobile industry. The success of the firm has been highly related to its supply chain management system. The relatively low prices of the firm’s products, compared to the products of competitors, is another factor that has highly benefited the performance of Toyota worldwide. In Qatar, Toyota has followed similar goals and strategies. The history of Toyota in Qatar are presented and evaluated. Also, reference is made to the automotive industry of Qatar, at the level that the external environment can highly influence organizational performance. It is revealed that the prospects for Toyota in Qatar are significant. However, it is necessary for the organization to review its strategic framework periodically ensuring that the competitiveness of the firm towards its rivals is kept at high levels. A brief history of Toyota Toyota Motor was established in Japan in 1937 (Toyota Corporation 2012, History). Toyota Motor Corporation has resulted from the merge between Toyota Motor Co and Toyota Motor Sales Co in 1982 (Toyota Corporation 2012, History). Through the decades the firm established production units worldwide; still, the firm’s critical strategic decisions have been traditionally developed in Japan. Of particular importance have been the organization’s production units in USA, established in 1988, in UK, established in 1992 and in China, established in 2000 (Toyota Corporation 2012, History). Toyota Corporation focuses on the ‘Motor Vehicle Production and Sales’ (Toyota Corporation 2012, Overview). In 2011, the firm’s employees worldwide were estimated to 317,716 (Toyota Corporation 2012, Overview). In 2011, the firm’s profits reached the 18,993.6 (in billion yen), slightly increased from 2010, when the firm managed to achieve a profit of 18,950.0 (in billion yen, Toyota Corporation 2012, Overview). In 2009 the firm’s profits were estimated to 20,529.5 (in billion yen, Toyota Corporation 2012, Overview). In other words, the firm faces delays in regard to its profitability. This problem is made clear by reviewing the firm’s performance for the years 2006 to 2010 in US and Europe (Graph 1). Still, the performance of the firm in other markets is quite encouraging. For example, reference can be made to the case of China and Brazil (Graph 2). Graph 1 – Toyota Corporation in USA and Europe, for 2006-2010 (Source: Toyota Corporation 2012, Figures) Graph 2 - Toyota Corporation in China and Brazil, for 2006-2010 (Source: Toyota Corporation 2012, Figures) How it all started in Qatar The presence of Toyota in Qatar is closely related to Abdullah Abdulghani & Bros. Co. (AAB), a firm that was established in 1958 (Qatar 40 Years Organization 2012, Attendees).

Tuesday, November 19, 2019

Clinical objectives Essay Example | Topics and Well Written Essays - 250 words - 5

Clinical objectives - Essay Example The calculation changes on the yearly basis, however, it is crucial for hospitals to stay current on the measures which are included and how their performance on the measures during the defined performance period will contribute to the overall total performance (TPS). To improve the quality of healthcare delivery, it is crucial to ensure that there is patient satisfaction. Hospital Impatient Value-Based Purchasing (HIVBP) program, and the Medicare reimbursements are closely linked to patient satisfaction. Patients have high expectations in their care and the attitude of care givers which greatly contributes to satisfaction. In a period where physicians are being measured by the quality of their treatment, it is crucial to understand the concept of value base purchasing and Medicaid reimbursement. Value-Based Purchasing (HIVBP) program and the Medicare reimbursements are closely linked to patient

Sunday, November 17, 2019

Charles Beard Essay Example for Free

Charles Beard Essay Charles Beard’s book, An Economic Interpretation of the U.S. Constitution, was published in 1913 and soon became one of the most controversial literary works of its time. Beard’s main thesis in this book is essentially that the Founding Fathers chose the specific format of the Constitution of the United States to protect their personal financial interests. Beard then goes on to argue that the Constitution was written by an â€Å"elite† attempting to safeguard their own assets and financial status. Beard was expanding on Carl L. Becker’s thesis of class conflict. In the eyes of Beard, the Constitution was created by the Founding Fathers as a â€Å"counter revolution† that ran against the wishes of farmers and laborers. Beard’s theory and his publication of this book were so controversial because it seemed to demean the Constitution and everything it stood for, which angered politicians and most of the legal community, at least those who didn’t ignore it completely. However, many historians and history professors seemed to recognize it and accept it. Many people were angered by Beard’s theory because, since it disparaged the Constitution and Founding Fathers, it seemed to put down almost everything our country stands for. Taking a sacred piece of history that started our nation and trampling on it by saying that it was nothing more than rich men trying to save their bank accounts and put down the common man is bound to upset more than just a few people. One of Beard’s main critics of the 20th century is a man named Forrest McDonald. McDonald uses his study of the voting habits and financial and economic concerns of the delegates at the Constitutional Convention in Philadelphia to prove that their motives were not financial-based. He asserts that the majority of the delegates who had public securities were Antifederalists. He concludes his study by saying, â€Å"Anyone wishing to rewrite the history of those proceedings largely or exclusively in terms of the economic interests represented there would find the facts to be insurmountable obstacles†. What McDonald means by this is that Beard, who was attempting to â€Å"rewrite the history† of the proceedings of the Constitutional Convention, was doing so without any actual evidence and therefore had an empty argument. I believe that Beard’s thesis, although an interesting notion, was not necessarily a valid argument and did nothing except anger most people in the political community. Even though many people in the late 20th century accepted his thesis, I disagree with it. My reasoning for this is that, although the Founding Fathers were economically prosperous, I believe that their status made them the only ones that could be able to write the Constitution and who were in a position to make the most crucial moves in establishing the United States. I do not believe that the Founding Fathers created the Constitution simply protect their financial status, I believe their status was the reason they could write the Constitution.

Friday, November 15, 2019

Arthur Dimmesdale and John Proctors Guilt and Sin Essay -- The Crucib

Arthur Dimmesdale and John Proctor's Guilt and Sin Guilt is something that weighs heavily on the human soul. It incorporates itself in our dreams, our thoughts, and our actions. Everywhere we turn, it stares us blankly in the face. While it is unbearable to suffer, guilt is an emotion that reaffirms our humanity. Repentance of a particular guilt, being spiritual, physical or both, is evidence that we are beyond the baseness of our animal tendencies. This fact has not gone unnoticed to the many great figures of literature. They have explored the sentiments of guilt and repentance by exploiting the conscience of flawed characters. In The Scarlet Letter, Nathaniel Hawthorne presented to the world Reverend Arthur Dimmesdale, a man suffering in a past sin. Likewise, in his play The Crucible, the great modern playwright, Arthur Miller, penned the character of John Proctor to allegorize the dangers of moral passivity. Their guilt and repentance were the primary causes of their â€Å"undoing†. Dimmesdale and Proctor were both martyrs to their sin. More specifically, they were both martyrs to the sin of adultery. Being a man of the cloth, this was especially painful for Dimmesdale. How could â€Å"a ruined soul like [his] effect toward the redemption of other souls?† (Hawthorne 182). As he confessed so mournfully to Hester, his partner in sin, â€Å"Canst thou deem it, Hester, a consolation, that I must stand up in my pulpit and meet so many eyes turned up to my as if the light of heaven were beaming from it†¦and then look inward, and discern the black reality of what they idolize?†(Hawthorne 182). He was so consumed by his hypocrisy that he turned to self-masochism as a means of escape. In stark contrast to Hester’s outward ... ...e†(Miller 22). All he wanted to do was get on with his farming and continue to live happily with his wife. It was only when the witch hunt directly affected him did he realize the gravity of his mistake. This was completely different to Dimmesdale’s seven long years of suffering. However, unlike Dimmesdale, when faced with the decision to confess and live or stand by his convictions and die, Proctor’s love for life interfered. He had so much to live for including his children and his livelihood. Only his honor steered him back to the importance of his cause. Arthur Dimmesdale and John Proctor were both martyrs for personal and societal guilt. They paid earthly penances and the final penance of death. Their â€Å"undoing† was a necessity for a society at the brink. Without their sacrifice, the society they lived in would have collapsed under its own weight.

Tuesday, November 12, 2019

Compare and Contrast the 2 Brothers in Sonnys Blues

The story, Sonny’s Blues, describes the lives of two brothers growing up in Harlem in the early 1960’s. Sonny and his brother are different in the way the go about life in general. They were both raised in the same household, yet they grew up to be totally different people. As the story progresses we see that both brothers have troubles in their lives and we get to see how each thinks and acts when facing such ordeals. While the brothers differ in the way they internalize and cope with their problems, they both show selfish characteristics, but ultimately feel remorseful for not being in each others’ lives.The narrator though an educator, is not very good at verbalizing his emotions. He tends to be the person who keeps everything inside and lets all his emotions turn to resentment. When he found out his brother had been arrested he still couldn’t verbalize how he felt knowing his brother was a junkie, â€Å"I couldn't believe it: but what I mean by that i s that I couldn't find any room for it anywhere inside me. I had kept it outside me for a long time. I hadn't wanted to know.I had had suspicions, but I didn't name them, I kept putting them away† (Baldwin). For many years, the narrator has been stuffing his emotions down, ultimately resulting in complete disassociation from situations for example his mothers and daughters death Also after finding out that Sonny was arrested, he sees a kid that was friends with Sonny and automatically has anger for him even though realistically he had nothing to do with Sonny’s arrest, â€Å"But now, abruptly, I hated him. I couldn't stand the way he looked at me† (Baldwin).That narrator is unable to cope and deal with problems as they come, instead he acts like a child, sort of folding his arms and giving the silent treatment or automatically jumping to conclusions to put himself on a pedestal. Sonny runs away from his problems, both mentally and physically. Even though he is fa cing his problems head on as opposed to the brother who sweeps things under the rug, he does it in a destructive manner. Physically he ran away from Harlem to get away the stereotype of that area. At first he turns to music to fix his problems, and then heroin.Sonny left school, and joined the navy to get as far away from Harlem as he possibly could. When Sonny returns from prison, he tried explaining to his brother what music does for him, â€Å"†It's not so much to play. It's to stand it, to be able to make it at all. On any level†(Baldwin). He frowned and smiled: â€Å"In order to keep from shaking to pieces. â€Å"† He didn’t want to be a prisoner of Harlem anymore, but became a prisoner to heroin. At first Sonny did not feel that heroin was necessarily a bad thing, â€Å"†It makes you feel-in control.Sometimes you've got to have that feeling† (Baldwin). Sonny feels that even while all doped up on heroin, he feels in control of his life a nd his circumstances. Even though Sonny takes on different approaches in finding sense in his life; whether through the army, music, or heroin, they do not realistically solve any of his problems they just mask his confusion and indecision temporarily. In my opinion the narrator, is a very selfish man, he only cares about his feelings and not those around him.The narrator broke his promise to his mother to take care of Sonny, â€Å"Two days later I was married, and then I was gone. And I had a lot of things on my mind and I pretty well forgot my promise to Mama until I got shipped home on a special furlough for her funeral† (Baldwin). The narrator’s mother wanted the boys to be close, because they will only have each other when their parents pass away. Also, the narrator has a very judgmental outlook on people, he does not let anyone state their opinion and even if he does he isn’t truly listening.In an argument with Sonny, â€Å"’I hear you. But you nev er hear anything I say. ’†Sonny is obviously upset that his brother doesn’t respect his wishes to pursue a music career, along with wanting to go to the navy. Both brothers leave home, the narrator when he gets married, and Sonny when he decides to pursue his life as a musician. Neither of them take into account their mother, or each other for that matter when they go on living their lives. They both feel guilty for not being as close to each other as they could have been.Sonny makes that clear in a letter he writes the narrator, â€Å"’Dear brother, You don't know how much I needed to hear from you. I wanted to write you many a time but I dug how much I must have hurt you and so I didn't write†¦and I swear if I'd known what I was doing I would never have hurt you so’† (Baldwin). Sonny feels bad for abandoning the family, and turning to drugs and even though he does it after sometime, he has the courage to say that he’s sorry for his actions.Towards the end of the story, when the brothers are conversing in the apartment, the narrator realizes how his silent treatment and cold demeanor has affected his brother. â€Å"I realized, with this mocking look, that there stood between us, forever, beyond the power of time or forgiveness, the fact that I had held silence-so long! -when he had needed human speech to help him† (Baldwin). Just from a look that Sonny gives him, he understands that Sonny needed his guidance and support through all these years. In conclusion, both brothers are on a pretty harmful way of solving their problems.The narrator lets things fester inside until he explodes. Then there is Sonny while yes, he is trying new ways to manage, at the end of the day is running away from his problems. Both brother’s only worried about themselves and their needs, neither take in to account their families the people that need them the most. Luckily towards the end of the story they both sort of come to a mutual understanding of each other. I think they both realize neither of them will really change how they are, and they both just need to be understanding of each other.

Sunday, November 10, 2019

Personal Statement: Who Am I?

Olivia in the dictionary, and you might find; noun, a female given name. From the Latin word olive. But this name doesn't define me. I could also tell you all the things I do; school, yearbook, HOSE President & Treasurer, but those don't define me either. I ask myself simple questions to find out who I really Am I the leader of a crowd, or a background follower? Do I direct groups or do I get directed? Am I the thinker or do I let others think for me? Am I part of the in- rood, or am I an outsider looking in?Who truly am l? To find the answer I look deep inside myself and discovered the truth. I am a uniquely defined Individual. I don't go to parties every weekend nor do I consider myself as weak minded. I am simply neutral in the world. I have friends of both sides of the spectrum and I feel free to talk to whomever I want regardless of what others around me think. I am bossy, and I personally hate people telling me what to do, I am a natural fighter. I fight for what I think Is rig ht, some may call me obelisks, but I keep the respect between adults and myself.The activities I listed above Just show that I am a hardworking-dedicated individual who doesn't quit at the first sign of trouble. I don't let the teacher who constantly looses my homework get me down; I Just work extra hard and redo all the work required of me. I am simply Olivia, and no one can take that away. People may tear me down emotionally but mentally I am stronger than anyone can Imagine. I am the girl defined by no one but her own standards, standards that continue to grow thicker every day.

Friday, November 8, 2019

4 Great Going Away Gifts for Coworkers

4 Great Going Away Gifts for Coworkers Ever wonder what to get a coworker who’s leaving your office? This person may have become a real friend outside of work. Or he or she might be a face to nod and smile at in the hallway, with whom you’ve spent very little time outside the office. Either way, it’s nice to commemorate the time you’ve spent working together. It’s just very often hard to think of what to get for the going away gifts. Maybe the most appropriate thing to do is band together as a team and chip in a little (say, $5-10) for a larger, more expensive group gift. If that turns out to not be the best option, you can consider individual gifts. Just keep in mind that whatever gift you give should be something they’d be comfortable with unwrapping in public, in a professional setting.Here are some great ideas for departure gifts.Gifts for the Coworker Moving AwayIf your coworker is relocating, particularly somewhere new where they don’t have roots, consider a gift rela ted to their new locale:  a book about the history or food culture of a place, a local guidebook or restaurant guide, etc. Or, you could try the sentimental route and get a commemorative photo or book or trinket from where you are currently, so they can take it with them to their new life somewhere else.Gifts for the Coworker  Changing CreersMaybe  your coworker is switching fields- or even careers. A gift to commemorate the new job is a lovely gesture of support. A little gift bag of useful items, trinkets, or aspirational things suited for their new job or role can be very much appreciated- particularly if you add a little note of support to send them off with confidence!Gifts for the Coworker  Who Found Success on the JobWas there a particular achievement you shared? Consider having your team find an object or souvenir that symbolizes your work together. Then frame it, or put it on a stand, and make sure to all sign it in permanent marker. Wherever your coworker goes, the y’ll have the memory both of the accomplishment and of your team to brighten their office.Gifts for the Coworker You Don’t Know That WellIf you don’t have the time or energy to put together one of these more specialized gifts, the old standbys are always available! Try a bouquet of flowers and a group-signed card on their desk. A bottle of champagne or their favorite wine. Or even simply slap a bow on a box of chocolates. Remember, it’s the thought that counts.

Tuesday, November 5, 2019

Stony Corals (Hard Corals) Facts

Stony Corals (Hard Corals) Facts Stony corals, also called hard corals (as opposed to soft corals, like sea fans), are the reef-builders of the coral world. Learn more about stony corals - what they look like, how many species there are, and where they live. Characteristics of Stony Corals Secrete a skeleton made of limestone (calcium carbonate).Have polyps that secrete a cup (calyx, or calice) in which they live, and in which it can withdraw for protection. These polyps usually have smooth, rather than feathery tentacles.Are usually transparent. The brilliant colors associated with coral reefs are not caused by the corals themselves, but by algae called zooxanthellae that live within the coral polyps.Are composed of two groups: the colonial corals, or reef-builders, and the solitary corals. Stony Coral Classification Kingdom: AnimaliaPhylum: CnidariaClass: AnthozoaOrder: Scleractinia According to the World Register of Marine Species (WoRMS), there are over 3,000 species of stony corals. Other Names for Stony Corals Stony corals are known by many different names: Hard coralsReef-building coralsHexacoralsHermatypic coralsScleractinian corals Where Stony Corals Live Corals arent always where you think theyd be. Sure, many of the reef-building corals are warm-water corals - restricted to tropical and subtropical areas where the water is salty, warm and clear. The corals actually grow faster when they have more access to the sun. They can build large reefs like the Great Barrier Reef in warmer waters. Then there are corals found in unexpected areas - coral reefs and solitary corals in the deep, dark sea, even as far down as 6,500 feet. These are the deep-water corals, and they can tolerate temperatures as low as 39 degrees F. They can be found around the world. What Stony Corals Eat Most stony corals feed at night, extending their polyps and using their nematocysts to sting passing plankton or small fish, which they pass to their mouth. The prey is ingested, and any waste is expelled out the mouth. Stony Coral Reproduction These corals can reproduce both sexually and asexually. Sexual reproduction occurs either when sperm and eggs are released in a mass spawning event, or by brooding, when only sperm are released, and these are captured by female polyps with eggs. One the egg is fertilized, a larva is produced and eventually settles to the bottom. Sexual reproduction allows coral colonies to form in new places. Asexual reproduction occurs through splitting, in which a polyp splits into two, or budding when a new polyp grows out of the side of an existing polyp. Both methods result in the creation of genetically identical polyps - and the growth of a coral reef. Asexual reproduction occurs through splitting, in which a polyp splits into two, or budding when a new polyp grows out of the side of an existing polyp. Both methods result in the creation of genetically identical polyps - and the growth of a coral reef.

Sunday, November 3, 2019

Recognizing Employee Contributions - Compensation & Benefits Essay

Recognizing Employee Contributions - Compensation & Benefits - Essay Example Every firm develops a specific structure of compensation and benefits. Incentive pay is an integral part of benefits being offered to employees. In this particular study views and opinions will be given to a new retail company so as to retain its talented base of employees. Team members tend to leave an organization when they are not satisfied with organizational environment or their compensation structure. It is major responsibility of HR manager to continuously modify compensation framework in order to deliver the best benefits to employees. Workforce can be motivated either through monetary rewards or non-monetary appreciation. This study outlines current scenario of a retail company that is into internet sales as well as has retail outlets. Employee survey in the firm has revealed that employees are not satisfied with compensation system and are leaving jobs. In this study different approaches would be highlighted that can help to retain employees and facilitate developing approp riate compensation system. Incentive pay is essential for retaining employees within the system. Pay can be defined as the first reward that is given to managers or employees. Incentives are additional benefits given to employees so as to motivate them for undertaking new challenges. Pay can be classified into three segments such as basic pay, pay for performance and merit pay. Basic pay occupies the main portion of compensation and is referred to as hourly wage or salary. Merit pay is totally based on predetermined criteria. On the other hand, pay for performance can be defined as incentive pay system. Rewards are given to distinguish between competencies possessed by team members and their respective contributions towards firm’s overall success. There are certain new pay techniques that can be adopted by the retail company so as to retain its employee base. Amongst all the techniques two mechanisms are most suitable in the given situation such as

Friday, November 1, 2019

DNA Sequencing Essay Example | Topics and Well Written Essays - 4000 words

DNA Sequencing - Essay Example The Sanger methods are able to sequence best from 30-350 nucleotides and therefore genomic sequencing strategies have been developed to sequence longer DNA of interest such as the gene of interest in this plant. In the shotgun sequencing strategies, DNA often of large size is shredded into smaller fragments that can then be sequenced individually. Shredding of the DNA is done by restriction enzymes or mechanically by shearing the DNA. The sequences of these fragments are then reassembled into their original order based on overlaps. Alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise duri ng assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in the assembly of â€Å"gaps†. Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. Pairwise-end sequencing is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun.... Usually alignment of the sequences is done by a computer program to yield the complete sequence. In Whole-genome shortgun, the DNA is obtained without prior physical map knowledge and indiscriminately sheared into fragments of 100kb which are then cloned into plasmids and transformed. The DNA inserts obtained from the plasmids are sequenced individually and consequently assembled into a long contiguous sequence. The strategy has limits due to gaps which arise during assembly due to the repeats in the sequences. Another strategy is primer walking which tends to deal with whole shortgun sequencing challenges in assembly of â€Å"gaps†. . Clones carrying inserts for sequences for both sides of the gap are identified and the DNA is sequenced normally. Resultant sequence is used to design a primer downstream from the former primer position. These steps are repeated over and over until the complete sequence of the insert is elucidated. Pairwise-end sequencing (double-barrel shortgun ) is another strategy for genome sequencing which is performed on both sides of DNA of interest as opposed to one in whole-genome shortgun. It reduces â€Å"gaps† thereby minimizing assembly errors which are common in whole-gun sequencing. However it poses a huge computational challenge during assembly. DNA is shredded into 150mb fragments and inserted into BACs in hierarchical shortgun sequencing strategy. Inserts are mapped into a physical map and organized by known location â€Å"Golden Tiling Path†. Inserts are fragmented further and cloned into plasmid where they are again recovered and sequenced according to â€Å"the Golden Tiling Path†. This strategy is applied for long pieces of DNA such as whole genome or chromosome and in